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School of Medicine Columbia

Confocal Imaging

Confocal microscopy, an advanced imaging technique, revolutionizes the way we observe biological specimens by selectively focusing on specific depths within the sample. By eliminating out-of-focus light, confocal microscopy enhances resolution and enables three-dimensional reconstructions, providing researchers with unparalleled precision in studying cellular structures and dynamic processes.

Available Equipment

The SP8 TCS Multiphoton system is a state-of-the-art platform equipped with:

  • Upright DM6000 CFS microscope
  • 10X Dry, 20X Dry, 25X Water immersion, 40X Water, 63X Oil, and X-Clarity 25X objectives
  • Two internal PMT detectors
  • Two internal HyD detectors
  • HyD-RLD high sensitivity detectors
  • 488nm, 552nm, and 638nm lasers visible lasers
  • Coherent Chameleon Ultra II 680-1080 tunable laser
  • LAS AF 3D analysis software


VA Building 9, access through IRF personnel only

IRF Contact

Anna Harper


  • UV, 5 Fixed Diode lasers 405nm, 448nm, 488nm, 514nm, 561nm, 638nm
  • HyD S detectors
  • Transmitted light 
  • 10X, 20X, 25X water, 40X oil, 63X oil objectives
  • Software package includes LASX 3D
  • Lightning Super Resolution Capabilities



IRF Contacts

Anna Harper

Coming Soon


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